Generator

Part:BBa_K2451004:Design

Designed by: Matthew Kubis   Group: iGEM17_UT-Knoxville   (2017-10-23)


SoxS Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with Gibson Assembly. The RBS is immediately flanked by the SoxS start codon, which could hamper expression.



Source

SoxS was obtained from e. Coli MG1655 genomic DNA. The backbone, RBS, and terminator were amplified from our 2016 part, BBa_K1966003.

References